Development of analytical methods for the determination of Early Biological Effects and Altered Structure and Function after Exposure to Carcinogenic and Mutagenic Compounds

Status:

ongoing

Aims:

Use of multiple genotoxicity tests to study DNA damage of chemicals in humans (in vivo) and in cell lines (in vitro). Improving methods to assess genotoxicity and mutagenicity of chemicals, e.g., developing high-throughput methods based on flow cytometry or automated methods based on imaging microscopy to assess chemically-induced mutagenic effects in exposed workers. Identifying early genotoxic events such as the formation of DNA adducts in vivo and in vitro.

Activities/Methods:

PAH-induced lung and bladder cancer, e.g., in coke oven workers

• Metal-induced lung cancer, e.g., in welders
• Aromatic amine-induced bladder cancer
• Imaging Microscopy
• Flow Cytometry

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